Analysing genetic roles of RNA-binding proteins and alternative splicing in Drosophila stem cells
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Project Leader
Professor Gary Hime+ 61 3 834 45796
Project Details
We have identified that the RNA-binding proteins, Musashi, Enhancer of Rudimentary and Held-Out-Wings (HOW) are required to either prevent stem cells from differentiating in the stem cell niche or are required to regulate the cell cycle of stem cells. This project utilises a variety of genetic, molecular biology and immunostaining techniques to identify the roles of these proteins in different stem cell populations.
Researchers
Nicole Siddall, Postdoctoral Researcher
Franca Casagranda, Research Assistant
Farnaz Shapouri, PhD student,
Shaghyegh Saeidi, PhD student
John Qi, MSc student
Collaborators
Professor Eileen McLaughlin, University of Auckland
Research Publications
- Monk AC, Siddall N, Volk T, Fraser BA, Quinn LM, McLaughlin EA, Hime GR. The RNA-binding protein HOW is required for stem cell maintenance in the testis and for the onset of transit amplifying divisions. Cell Stem Cell 2010; 6(4): 348-60.
- Siddall NA, McLaughlin EA, Marriner NL, Hime GR. The RNA-binding protein Musashi is required intrinsically to maintain stem cell identity. Proc Natl Acad Sci USA 2006; 103: 8402-8407.