Doherty Institute Flow Cytometry Facility
Welcome
Welcome to the Peter Doherty Institute Flow Cytometry Facility.
Our facility provides all researchers with a world class flow cytometry resource. Equipped with the latest high-end flow cytometric technology we provide a high quality cell sorting and cell analysis service for The University of Melbourne and broader scientific community.
Our facility offers services catering for animal, human, and non-mammalian cell and particle sorting in a PC2 environment, as well as catering for infectious sample sorting (e.g. HIV, HepC) in a dedicated PC3 environment.
For information regarding cell sorting and analysis capabilities, please see our main Melbourne Cytometry Platform page
Contact us for assistance in anything Flow Cytometry!
Location: Room 8042/43, Level 8
The Peter Doherty Institute for Infection and Immunity
The University of Melbourne
792 Elizabeth St
Melbourne Vic 3000, Australia
Facility email: micro-flowlab@unimelb.edu.au
Lab phone: (03) 8344 9937
Facility Manager: Dr Vanta Jameson
Email: vanta.jameson@unimelb.edu.au
Cytometry Technical Specialists:
Dr Alison Morey alison.morey@unimelb.edu.au
Mr Oliver Eltherington oliver.eltherington@unimelb.edu.au
Ms Tanvi Damani tanvi.damani@unimelb.edu.au
Melbourne Cytometry platform manager
Dr Alexis Gonzalez, alexis.gonzalez@unimelb.edu.au
Cytometry Links
- Australasian Flow Cytometry Group
- BD Biosciences
- International Society for Advancement of Cytology
- Purdue University Cytometry Laboratories
- ThermoFisher Fluorescence Tutorials
- Cytek Biosciences
Hours of Operation: Available to licensed users 24 hours 7 days a week
Cytek Aurora Spectral Cytometer
The Cytek Aurora is equipped with five lasers – UV (355nm), violet (405nm) blue (488nm), yellow (561nm) and red (640nm), three scattering channels, and up to 64 fluorescence detectors. Spectral traces of each fluorochrome are recorded and then unmixed in a highly multicolor experiment. As a result of this approach, combinations of fluorochromes that previously were incompatible to run together by conventional flow cytometric methods are now possible with this technology. As long as a fluorochrome’s spectral fingerprint is distinct from another they can be unmixed. This enables panel design of >36 colours possible.
Cytek Aurora
Lasers: 5 (355nm, 405nm, 488nm, 561nm and 640nm)
Fluorescent Detectors: 64 Channels
Tubes: Polystyrene
Software: Spectroflo
For information on common fluorochromes used on the Aurora click here.
Hours of Operation: Available to licensed users 24 hours 7 days a week
BD LSR Fortessa
The LSR Fortessa’s are equipped with four and five laser lines and are capable of detecting 18 parameters; 2 scatter and 16 fluorescent detectors. Check each instrument for the configuration and the facility staff are happy to advise on fluorochrome combination feasibility and necessary controls.
LSR Fortessa 1
Lasers: 4 (405nm,488nm, 561nm and 633nm)
PMT Detectors: 18 Fluorescent Channels
Tubes: Polystyrene
Software: FACSDiva
High Throughput Sampler (HTS) 96 well plates
Fast Analysis Rates >20,000 events per second
LSR Fortessa 2, 3 and 4
Lasers: 5 (355nm,405nm,488nm, 561nm and 633nm)
PMT Detectors: 18 Fluorescent Channels
Tubes: Polystyrene
Software: FACSDiva
High Throughput Sampler (HTS) 96 well plates
Fast Analysis Rates >20,000 events per second
FACS Canto II
Lasers: 2 or 3 ((405nm),488nm and 633nm)
PMT Detectors: 7-8 Fluorescent Channels
Tubes: Polystyrene
Software: FACSDiva
High Throughput Sampler (HTS)
See our main Melbourne Cytometry Platform - Doherty cell sorting page for cell sorting capabilities including optical configurations
Our cell sorters include:
PC2: BD FACS Aria III and Beckman Coulter CytoFLEX SRT conventional sorters and a Cytek Aurora CS spectral sorter
PC3: BD FACS Aria Fusion