Beckman Coulter CytoFLEX S
Specifications
The CytoFLEX S analyser has 4 lasers and 13 fluorescent detectors + FSC, SSC
This instrument also has a violet SSC detector for nano-particle analysis
Sample loading (manual) : 5ml tube, 1.5ml eppendorf tube, 1ml 'bullet' tube
Automatic plate loading : 96-well flat, U- or V-bottom plates
Software: CytExpert
Download the laser/ detector PDF here
Laser/ Filter combination | Fluorochromes |
---|---|
Violet (405nm): 450/45 | DAPI, BV421, Pacific Blue, V450, AF405, Cascade Blue, Dyecycle violet |
Violet (405nm): 525/40 | V500, AmCyan, BV510, Live/dead aqua, CFP |
Violet (405nm): 405 | 405 SSC |
Violet (405nm): 660/20 | BV650 |
Blue (488nm): 525/40 | FITC, AF488, GFP, CFSE, YFP, Dyecycle green, FAM |
Blue (488nm): 585/15 | DHE |
Y/G (561nm): 585/42 | PE, mOrange, AF568, dTomato, TAMRA |
Y/G (561nm): 610/20 | PE-Texas Red, PE-AF610, RFP, DsRed, mCherry, AF594, Texas Red |
Y/G (561nm): 690/50 | PE-Cy5, 7AAD, PI |
Y/G (561nm): 780/60 | PE-Cy7, PE-AF750, PE-vio770 |
Red (633nm): 660/20 | APC, AF633, AF647, APC-Cy5, Sytox Red dead |
Red (633nm): 712/25 | AF700 |
Red (633nm): 780/60 | APC-Cy7, APC-AF750, APC-H7 |
Additional filter sets by request:
V780/60 (BV786)
B510/20 (GFP/ YFP separation)
V610/20 (BV605)
V710/50 (BV711)
B690/50 (PerCP-Cy5.5)