Beckman Coulter CytoFLEX S



The CytoFLEX S analyser has 4 lasers and 13 fluorescent detectors + FSC, SSC

This instrument also has a violet SSC detector for nano-particle analysis

Sample loading (manual) : 5ml tube, 1.5ml eppendorf tube, 1ml 'bullet' tube

Automatic plate loading : 96-well flat, U- or V-bottom plates

Software: CytExpert

Download the laser/ detector PDF here

Laser/ Filter combination 


Violet (405nm):  450/45 DAPI,  BV421, Pacific Blue, V450, AF405, Cascade Blue, Dyecycle violet
Violet (405nm): 525/40 V500, AmCyan, BV510, Live/dead aqua, CFP
Violet (405nm): 405 405 SSC
Violet (405nm): 660/20 BV650
Blue (488nm): 525/40 FITC, AF488, GFP, CFSE, YFP, Dyecycle green, FAM
Blue (488nm): 585/15DHE
Y/G (561nm): 585/42 PE, mOrange, AF568, dTomato, TAMRA
Y/G (561nm): 610/20 PE-Texas Red, PE-AF610, RFP, DsRed, mCherry, AF594, Texas Red
Y/G (561nm): 690/50 PE-Cy5, 7AAD, PI
Y/G (561nm): 780/60 PE-Cy7, PE-AF750, PE-vio770
Red (633nm): 660/20 APC, AF633, AF647, APC-Cy5, Sytox Red dead
Red (633nm): 712/25 AF700
Red (633nm): 780/60 APC-Cy7, APC-AF750, APC-H7

Additional filter sets by request:

V780/60 (BV786)

B510/20 (GFP/ YFP separation)

V610/20 (BV605)

V710/50 (BV711)

B690/50 (PerCP-Cy5.5)