Overview

Welcome

Welcome to the Doherty Institute Flow Cytometry Facility.

Our facility provides all researchers with a world class flow cytometry resource. Equipped with the latest high-end flow cytometric technology we provide a high quality cell sorting and cell analysis service for The University of Melbourne and broader scientific community in and around Melbourne.

Our facility offers services catering for both animal and human cell sorting in a PC2 environment, as well as catering for infectious sample sorting (e.g. HIV, HepC) in a dedicated PC3 environment.

The facility currently houses three high speed cell sorters and nine analysers.

Acknowledgement of the Facility in publications

Core facilities and facility staff rely on acknowledgement in research publications. Acknowledgement is a measurement of relevance and importance of the technology/ facility to the completion of your research and is directly linked to funding of the facilities for instrument upgrades, new technology investment and research support staff.

Please acknowledge the Melbourne Cytometry Platform (Doherty Institute node) in your publications if you make use of any of the equipment in this facility. If appropriate, please also acknowledge, or include in authorship to specific facility staff for assistance during your experimental design process.

Contact us for assistance in anything Flow Cytometry!

Location: Room 8042/43, Level 8
The Peter Doherty Institute for Infection and Immunity
The University of Melbourne
792 Elizabeth St
Melbourne Vic 3000, Australia

Facility email: micro-flowlab@unimelb.edu.au
Lab phone: (03) 8344 9937

Facility Manager: Tina Luke
Emailtluke@unimelb.edu.au
Office phone: (03) 90358171

Research Officers 

Melbourne Cytometry platform manager

Dr Alexis Gonzalez, alexis.gonzalez@unimelb.edu.au

Staff

  • photo of Tina Luke
    Tina Luke

    Facility Manager

  • photo of Angela Hind
    Angela Hind

    Flow Cytometry Operator

  • photo of Catherine Li
    Catherine Li

    Flow Cytometry Operator

  • Lankesha Yapa
    Lankesha Yapa

    Flow Cytometry Operator

Doherty Institute Flow Cytometry Facility Prices

Instrument/Service

School of Biomedical Sciences

Other Academic

Commercial

Cytek Aurora 5 Lasar$50$55Contact manager
FACS Canto II analyser$50$55Contact manager
LSR II analyser$50$55Contact manager
LSR Fortessa analysers$50$55Contact manager
Assisted cell sorting$100$115Contact manager
Unassisted cell sorting$50N/AN/A

Bookings and Training

Once training is complete access will be granted to book instruments.

We also assist with panel design and data analysis/interpretation.

Click the following link to register for access to the facility:

https://cytometry-um.calpendo.com/#calendar

Booking Policy

Cell sorter bookings via the online booking system, Calpendo, must be made at least 24 hours in advance of required service. For bookings with less than 24 hours notice, please contact the manager to check availability.

Users can cancel their bookings via the online booking system up to 48 hours before their sort.

Cancellations with between 24-48 hours notice will be charged 50% of the booked time, minimum of 1 hour. Cancellations with less than 24 hours notice, and no-shows will be charged in full.

Minimum booking is 1 hour after which users will be charged in 30-minute increments.

All cells for sorting must be sieved through mesh, preferably 40 µm, prior to sorting.

Charging starts from the time booked, so it is in your interest to arrive on time.

For the full booking and conditions policy click here.

Data analysis

The Melbourne Cytometry Platform offers FlowJo 10 and FCS Express site licences for University staff and students, as well as our affiliate organisations. How to register?

  • FlowJo License: Please view our Knowledge Article for licensing details and fill out a Software Installation form if you are interested in purchasing a license.
  • FCS Express License: Please contact Vanta Jameson for license details and registration instructions.

Cytometry Links