Overview

Welcome

Welcome to the Doherty Institute Flow Cytometry Facility.

Our facility provides all researchers with a world class flow cytometry resource. Equipped with the latest high-end flow cytometric technology we provide a high quality cell sorting and cell analysis service for The University of Melbourne and broader scientific community in and around Melbourne.

Our facility offers services catering for both animal and human cell sorting in a PC2 environment, as well as catering for infectious sample sorting (e.g. HIV, HepC) in a dedicated PC3 environment.

The facility currently houses three high speed cell sorters and seven analysers.

Contact us for assistance in anything Flow Cytometry!

Location: Room 8042/43, Level 8
The Peter Doherty Institute for Infection and Immunity
The University of Melbourne
792 Elizabeth St
Melbourne Vic 3000, Australia

Facility email: micro-flowlab@unimelb.edu.au
Lab phone: (03) 8344 9937

Facility Manager: Tina Luke
Emailtluke@unimelb.edu.au
Office phone: (03) 90358171

Research Officers 

Melbourne Cytometry platform manager

Dr Alexis Gonzalez, alexis.gonzalez@unimelb.edu.au

Staff

  • photo of Tina Luke
    Tina Luke

    Facility Manager

  • photo of Angela Hind
    Angela Hind

    Flow Cytometry Operator

  • photo of Catherine Li
    Catherine Li

    Flow Cytometry Operator

  • Lankesha Yapa
    Lankesha Yapa

    Flow Cytometry Operator

2018 Doherty Institute Flow Cytometry Facility Prices

Instrument/Service

School of Biomedical Sciences

Other Academic

Commercial

FACS Calibur analyser$30$35Contact manager
FACS Canto II analyser$50$55Contact manager
LSR II analyser$50$55Contact manager
LSR Fortessa analysers$50$55Contact manager
Assisted cell sorting$100$115Contact manager
Unassisted cell sorting$50N/AN/A

Bookings and Training

Once training is complete access will be granted to book instruments.

We also assist with panel design and data analysis/interpretation.

Click the following link to register for access to the facility:

https://cytometry-um.calpendo.com/#calendar

Booking Policy

Cell sorter bookings via the online booking system, Calpendo, must be made at least 24 hours in advance of required service. For bookings with less than 24 hours notice, please contact the manager to check availability.

Users can cancel their bookings via the online booking system up to 48 hours before their sort.

Cancellations with between 24-48 hours notice will be charged 50% of the booked time, minimum of 1 hour. Cancellations with less than 24 hours notice, and no-shows will be charged in full.

Minimum booking is 1 hour after which users will be charged in 30-minute increments.

All cells for sorting must be sieved through mesh, preferably 40 µm, prior to sorting.

Charging starts from the time booked, so it is in your interest to arrive on time.

For the full booking and conditions policy click here.

Data analysis

The Melbourne Cytometry Platform offers a Flowjo 10 site licence for University staff. Please contact Dr. Alexis Gonzalez for pricing and other enquiries.

Melbourne Cytometry Platform Nodes

Other Cytometry Facilities in the Melbourne Research Community

Instrumentation for Flow Cytometry is a significant capital investment and as members of the broader medical research community here in Melbourne, we want to see such facilities fully utilised. If the Doherty Institute Flow Cytometry is fully booked for the time of planned use, the following facilities are available for use by our staff and students. In each case you should get in touch with the contact person of the facility to arrange this.

Additional University of Melbourne Flow Cytometry Platform Facilities and Resources

MBC Facility at Stem Cells Australia

Melbourne Brain Centre Flow Cytometry Facility

http://biomedicalsciences.unimelb.edu.au/departments/anatomy-and-neuroscience/research/services/melbourne-brain-centre-mbc-flow-cytometry-facility

Contact: Dr Vanta Jameson or Joshua Kie

Phone: 9035 8536 or mobile 0401 994 897

Email: mbc-flowlab@unimelb.edu.au

Equipment: BD FACS AriaIII (sorter), Fortessa (Medicine building), Beckman Coulter CyAn and CytoFLEX S (analysers)


Department of Medicine

Royal Melbourne Hospital

Phone:  +61 3 8344 3291

Equipment:

Beckman Coulter Cyan (2 lasers, 7 fluorescence detectors).

Contacts:

Dr Marian Todaro: mtodaro@unimelb.edu.au

Kevin Leeming-chin.lee@unimelb.edu.au


Biochemistry and Molecular Biology

Bio21

Phone: +61 3 83442534

Equipment:

BD FACSCanto II

Contacts:

Dr Yee-Foong Mok: ymok@unimelb.edu.au


Melbourne Dental School

The Royal Dental Hospital of Melbourne

Phone: +61 3 9341 1567

Equipment:

Beckman Coulter MoFlo XDP High speed Cell sorter (488 nm, UV, 633 nm).

Beckman Coulter FC500,

Beckman Coulter Quanta MPL (well suited for electrical volume (cell size) and microbial flow cytometry)

MoFlo Astrios cell sorter

Contacts:

Dr Alexis Perez Gonzalez: email – alexis.gonzalez@unimelb.edu.au

Dr Neil O'Brien Simpson: email - neil.obs@unimelb.edu.au


Materials Characterisation and Fabrication Platform (MCFP)

Chemical and Biomolecular Engineering Building

Phone: +61 3 8344 0176

Equipment:

Apogee A-50 Microflow Cytometer (small particles > 100 nm)

Partec Cyflow space

CyTOF

Contacts:

Paul Brannon: paul.brannon@unimelb.edu.au

Andrew Mitchel: andrew.mitchell1@unimelb.edu.au

More information: http://nanomaterials.unimelb.edu.au/content/flow-cytometry

Cytometry Links

Link to Scripps Logo in composite imageLink to ACS logo in composite imageLink to Purdue University logo in composite imageLink to ISAC logo in a composite image