Introduction to fluorescence microscopy, flow cytometry and histology
This lecture series will provide researchers with an introduction to Histology, Fluorescence Microscopy and Flow Cytometry applications. It is aimed at researchers who are new to Histology, Fluorescence Microscopy and Flow Cytometry applications, or those who would like to refresh their understanding. This event is free to attend and open to all.
Given the possible synergies, this 'Intro to' lecture series will be run by the Biological Optical Microscopy Platform (BOMP), Melbourne Cytometry Platform (MCP), and Melbourne Histology Platform (MHP) at The University of Melbourne.
This is an online event provided via Zoom. Zoom link will be provided once registered (Register here)
A full breakdown of the lectures is shown below:
| Date | Lecture | Platform |
|---|---|---|
| 10am-12pm Monday 3rd May | Lecture 1: Intro to Fluorescence and Fluorescence Microscopy | BOMP |
| 10am-12pm Tuesday 4th May | Lecture 2: Introduction to Flow Cytometry and examples of applications | MCP |
| 10am-12pm Wednesday 5th May | Lecture 3: Experimental design, acquisition and analysis on Fluorescence Microscopy | BOMP |
| 10am-12pm Friday 7th May | Lecture 4: Experimental design, acquisition & analysis on Flow Cytometry | MCP |
| 10am-12pm Monday 10th May | Lecture 5: Introduction to Histology | MHP |
| 10am-12pm Tuesday 11th May | Lecture 6: Experimental design for and applications in histology | MHP |
Lecture 1: Intro to Fluorescence and Fluorescence Microscopy (BOMP)
- Fluorescence
- Fluorescence tags, dyes & proteins
- Fluorescence Microscope
Lecture 2: Introduction to Flow Cytometry and examples of applications (MCP)
- Cytometry Platform: Who, what & where?
- Cytometer architecture
- Instrument subsystems: Fluidics, Optics & Electronics
- Nature of the signals (Light Scatter, Fluorescence)
- Pulse processing
- Data Visualisation
- Examples of applications in Cytometry
Lecture 3: Experimental design, acquisition and analysis on Fluorescence Microscopy (BOMP)
- Fluorescence Microscopy Applications
- Experimental design
- Digital imaging and Image analysis
Lecture 4: Experimental design, acquisition & analysis on Flow Cytometry (MCP)
- Experimental parameters in Cytometry
- Before you start
- Sample preparation
- Instrument setup
- Compensation and spectral unmixing
- Panel Design
- Experimental Controls
- Data acquisition and analysis
- Examples of artefacts
- Cell Sorting
Lecture 5: Introduction to Histology (MHP)
- The aims of histology
- Overview of the key steps of histology
- Broad options for sample fixation, embedding, sectioning, and staining
- Basic introduction to immunostaining
Lecture 6: Experimental design for and applications in histology (MHP)
- Integrating histology into experimental design
- Technology in sample preparation and staining
- Examples of applications of histology in biomedical and biological research