Stem Cell Disease Modelling Laboratory (SCDML) Phenomics Australia Node

Who We Are

Stem Cell Disease Modelling Laboratory is a service node of Phenomics Australia. We provide on-demand reprogramming of patient samples into induced pluripotent stem cells (iPSCs), their quality control and banking, focusing on the production of large-scale iPSC cohort. We can reprogram from various primary cell types, and with different reprogramming approaches. Our service also includes training of researchers in these procedures.

The platform is open to academic researchers and industry partners Australia-wide on a fee-for-service basis.

Our Services

• Reprogramming patient blood/ skin samples using episomal vectors to generate polyclonal iPSCs
• Monoclonal lines can be generated on request
• Quality control assessment of generated lines:
  • analysis of pluripotency markers (immunocytochemistry)
  • analysis of tri-lineage differentiation ability (immunocytochemistry)
  • analysis of colony morphology
  • mycoplasma testing
  • karyotyping (DNA extraction for SNP array)
  • STR analysis can be arranged on request
• Expanding the reprogrammed lines for 8 passages
• Supply 10 vials per reprogrammed line (5 at p0/p1, 5 at p8)
• PBMC isolation can be provided on request
• Tailored training in cell culture techniques for researchers entering the field
• Expansion of existing iPSC lines to share with other researchers
• Assistance with experimental planning
• Assistance with budget preparation for funding applications
• Assistance with amending ethics applications for funding applications
• Assistance with preparation of material transfer agreement documentation

Other Platforms

We work closely with other platforms part of Phenomics Australia - a network of forward-thinking research infrastructure providers enabling research discovery and high-impact healthcare outcomes in precision medicine. Through openly accessible service delivery centres, we offer specialised infrastructure, research services and technical expertise dedicated to advancing our fundamental understanding of health and disease and enabling next-generation innovations in healthcare and therapeutic development to benefit all Australians. We are supported by the Australian Government through the National Collaborative Research Infrastructure Strategy, (NCRIS). NCRIS supports Australia’s research capability by investing in research infrastructure and making it accessible to researchers across the nation.

Phenomics Australia provides a broad panel of in vivo and in vitro genome engineering and disease modelling, functional genomics and high-throughput screening, biobanking and pathology services and technical expertise, for discovery and a new understanding of the molecular basis of disease to benefit all Australians.

Read more about Phenomics Australia services