Cell sorting Doherty

Peter Doherty Institute cell sorting

The Peter Doherty Institute cytometry facility is the largest of the MCP nodes, hosting 4 cell sorters including 3 conventional cytometers one full-spectrum 5 laser Aurora CS for large panel or highly autofluorescent samples.

Staffed by 5 full-time cytometry technical specialists and managers, our staff are expert in multicolour immune cell sorting including from solid organs such as skin and lung. Our expertise extends to other cell and particle types, including tumours, cell lines, microbiology, marine and plant samples.

Our facilities are approved for PC2-level infectious, OGTR and biosecurity samples that can be sorted under aerosol management or within biosafety cabinets.

We also have the facilities to cater for the more infectious samples with cell sorters located in a Biosafety Cabinet and in the PC3 High Containment facility specialising in HIV and SARS-Cov2 cell sorting.

For enquiries, please email us

or call 8344-9937

PC2 Becton Dickinson FACS Aria III (5L)

For large, multi-colour panel sorting, including UV dyes and up to 6 violet dyes. Includes an air management system for samples categorised as PC2+ (BSL2+)

Aurora 5L CS

PC2 Aurora 5L CS spectral sorter

This full spectrum 5 laser, 64 detector cell sorter is available for large-panel or highly-autofluorescent sample sorting. 6-way sorting into eppendorf or 5ml tubes and plate sorting. Available in 70um, 85um, 100um and 130um nozzles.

The Aurora CS is within a class II biosafety cabinet

SRT

PC2 Beckman Coulter CytoFLEX SRT (4L)

This 4 laser 15 colour cell sorter in the PC2 lab is within a class II biosafety cabinet, specifically for the sorting of samples up to Risk 2 biohazard containment level. Equipped with a 100um nozzle, this instrument sorts 4 ways into 1.5ml or 5ml tubes, or 2 ways into 15ml tubes. Also for sorting into plates and slides.

The SRT is available for DIY after extensive training

PC3 Becton Dickinson FACS Fusion (4L)

For infectious human samples including Hepatitis, virulent influenza, SARS-CoV-2 and HIV, PC3 sorting is available on level 6.

The PC3 BD Aria Fusion cell sorter can only be booked by selected users after building induction, PC3 training and clearance. Samples sorted in the PC3 facility cannot be taken back to PC2 labs.