Alexis Perez Gonzalez, PhD
Cytometry Platform and Dental School node Manager
B. Biochemistry (summa cum laude), The University of Habana, Cuba (1996)
MSc Immunology, Centre of Molecular Immunology (Havana, Cuba) (1999)
PhD Biology, The University of Habana, Cuba (2003)
Alexis’ early research career in Havana, Cuba, and the basis of his PhD, focused on the molecular characterization and evaluation of monoclonal antibodies with anti-cancer properties in pre-clinical models. Upon relocation to Lisbon, Portugal to begin a postdoctoral career at the Gulbenkian Institute of Sciences, he was given the opportunity to manage the Flow Cytometry facility (2004-2006). In 2006 he joined the Flow Cytometry Core Facility (FCCF) at the European Molecular Biology Laboratory (EMBL, Heidelberg, Germany), acting first as a senior technical officer, then as manager of the facility (2012-2016). The EMBL years cemented Alexis’s passion for cytometry’s theory and practice. For a decade, he worked with and solved technologically challenging cutting-edge molecular biology projects involving rare populations and cellular markers, very low levels of fluorescence, complex biological samples and demanding downstream processes. In 2016, Alexis was appointed manager of the Melbourne Cytometry Platform (MCP) where he has overseen the expansion of the investment in state-of-the-art instrumentation and analysis resources, industry engagement and collaboration, education and the high-level support of over 350 university students and staff annually. As head of the MCP, Alexis applies his passion for and deep knowledge of instrument quality and performance characterisation methods to best match research challenges with instrument requirements.
Doherty Institute node manager
B. App Sci (Biochemistry/ Chemistry), Swinburne University of Technology (1998)
Upon completion of her university studies, Tina embarked upon a 10-year research assistant career involving many projects requiring flow cytometry, and gained valuable experience with a variety of different cell sorter and analyser instrumentation. She decided to pursue a Flow Cytometry career full time and in 2007 joined the Flow Cytometry Core Laboratory at Cancer Research UK, London. Here, she spent the proceeding four years further developing her skills in cytometry under the expert tuition of Mr Derek Davies. Tina was appointed in her current role as the manager of the Doherty Flow Cytometry Facility in 2011. Over the last decade, she has overseen the rapid expansion in capacity and state-of-the-art capabilities of the Doherty facility instrumentation and service. Tina has over 20 years of Flow Cytometry experience and can advise on multicolour panel design, analysis troubleshooting and experiment optimisation.
Vanta Jameson, PhD
MBC node manager
BSc (Hons) Biochemistry and Molecular Biology/ Genetics, The University of Melbourne (2000).
PhD, The University of Melbourne, Peter MacCallum Cancer Institute (2006).
Vanta's PhD in adult haemopoietic stem cell biology, first exposed her to the power of flow cytometry as both an analytical and cell recovery tool. Her studies characterised the expression of angiotensin converting enzyme (ACE) throughout human haemopoietic ontogeny and the function of ACE and the renin-angiotensin system in the murine haemopoietic system. Commenced in the early 2000's, these studies pushed the limits of flow cytometry instrumentation, with large, multi-colour antibody panels and dyes employed for flow cytometric analysis and sorting. These experiences resulted in the appreciation of the crucial nature of sample and experimental optimisation and controls. Following her PhD, she joined the Australian Stem Cell Centre and worked on murine embryonic stem cell (ESC) differentiation toward various mesenchymal fates, before commencing a post-doc in 2007 at the Monash Immunology and Stem Cell Laboratories (MISCL). Her investigations at MISCL again involved almost daily multi-colour flow cytometric analysis and sorting of human ESC-derived blood progenitor cells, and she quickly also became the resident lab educator of newcomers to flow cytometry. Her love of flow cytometry and teaching prompted a move into core facility management. Vanta has been the manager of the MBC flow cytometry node since its inception in 2012. With over a decade's first-hand experience as a medical research scientist and 9 years managing a core flow cytometry facility, Vanta appreciates the effort involved in sample preparation, in 'getting it right', and believes in making the process of flow cytometry as stress-free as possible for her facility's users. With experience in samples and research areas as varied as nanoparticles, stem cells, brain tissue, other solid organs and immunology, she is passionate about experimental optimisation to achieve robust, reproducible and publishable data. Contact Vanta for assistance with experimental and panel design, sample preparation and optimisation, data interpretation and publication readiness, instrument and software training.
Operator, Doherty Institute
Diploma of Laboratory Technology (Microbiology and Biotechnology), Box Hill Institute (2003)
Angela started her career working in molecular genetics at the Victorian Clinical Genetics services (VCGS/ Murdoch Children's Research Institute) operating sequencing and genotyping instrumentation before taking up a position as an operator at the WEHI Flow Cytometry facility. Angela worked under the expert guidance of Dr. Frank Battye for 7 years, gaining valuable experience operating and troubleshooting various cytometers before joining the Doherty Flow Cytometry Facility in 2012. Having worked in the field of flow cytometry for over 15 years, Angela has a lot of experience operating and troubleshooting cell sorters and benchtop analysers including the newly acquired spectral analyser Cytek Aurora. Angela has extensive experience setting up large multi-colour sorts from various cell types for bulk and single cell applications.
Operator, Doherty Institute
BSc Applied Medical Laboratory Sciences, RMIT (1992)
In 2001 Catherine started out managing a small confocal microscopy facility at La Trobe University before moving on to operate a FACS Aria cell sorter at St Vincent's Institute for Medical Research in 2005. 3 years later Catherine moved on to the Ludwig Institute for Cancer Research to set up a small Flow Cytometry facility before taking on the role as an operator at the Doherty flow Cytometry Facility in 2012. Working in the field of flow cytometry for over 15 years, Catherine is very experienced at setting up large multi-colour panel sorts from various cell types isolated from tissue and blood for bulk and single cell applications along with troubleshooting any instrument problems that arise.
Operator, Doherty Institute
BSc Biochemistry, University of Auckland, New Zealand (1998)
MSc, University of Auckland, New Zealand (2001)
On completion of her Masters, Lankesha worked for Genesis Research and Development Corp doing sequencing before moving to Australia to join the Australian Genome Research Facility (AGRF) to assist with the Wallaby Genome Sequencing project. In 2005 Lankesha took up the position of operator at the WEHI Flow Cytometry facility working under the tutelage of Dr. Frank Battye. Here she gained valuable experience in the operation and troubleshooting of many cell sorters and benchtop analysers. Lankesha joined the Doherty Flow Cytometry Facility as an operator in 2016. Having worked in the field for over 15 years, Lankesha sets up multi-colour sorts from a variety of different cell types for bulk and single cell applications, ensuring the best possible outcome for the Researcher.
Operator, Melbourne Brain Centre
BSc (Hons) Neuroscience/ Biochemistry and Molecular biology, The University of Melbourne (2010)
Lidia completed her Honours in behavioural neuroscience, investigating fear-learning and memory, before taking on a research assistant role and lab managing at a University of Melbourne visual neuroscience lab. Here, she worked independently to optimise sample preparation for flow cytometry and imaging. Lidia recently joined the MBC flow cytometry node and brings with her almost a decade of lab experience. She is highly motivated and keen to assist and advise researchers at all stages of their experimental setup to enable the output of high quality data.