Metabolic reprogramming of skeletal muscle stem cells

Project Details

One of the most important and unanswered questions in stem cell biology is how decisions regarding cell fate are regulated, specifically whether a cell undergoes self-renewal or commitment to a specific lineage. While the better part of the 20th century focused on the importance of cellular metabolism for the generation of energy, recent work has uncovered an essential role for metabolism in the generation of the building blocks (nucleotides, phospholipids, and amino acids) required by rapidly dividing cells.

Additionally, the metabolite balance of both stem and differentiated cells has been found to directly influence the epigenome through post-translational modifications of histones, DNA and transcription factors and therefore has important implications for stem cell lineage progression.

The overall goal of research into the link between metabolism and stem cell identity is to improve stem cell transplantation and regenerative medicine, nuclear reprogramming, transdifferentiation, and stable ex vivo expansion of stem cells.

This project will utilise cutting-edge single cell RNAseq techniques in collaboration with researches at the Walter and Eliza Hall Institute, and will have broad application in the fields of regenerative medicine, synthetic biology and cellular agriculture

Researchers


Dr Kate Murphy
, Senior Research Fellow

Dr James Ryall, Senior Research Fellow

Dr Kristy Swiderski, Senior Research Fellow

Dr Justin Hardee, Postdoctoral Research Fellow

Dr Marissa Caldow, Senior Research Fellow

Audrey Chan, Research Support Officer

Jennifer Trieu, Research Assistant

Timur Naim, Research Assistant

Dylan Chung, Research Support Officer

Alaina Lee, Senior Research Assistant

Suzannah Read, Research Assistant

Students

Savant Thakur, PhD student

Francesca Alves, PhD student

John Nguyen, Masters student

Chloe Li, Masters student

Aundrea Quek, Masters student

Yichen Xue, Honours student

Funding

2019-2021 ARC Discovery Project. Mechanisms of age - related changes in amino acid signaling in skeletal muscle

2018-2021 NHMRC Project Grant. Rescuing the Dystrophin-Glycoprotein Complex to protect muscles from wasting conditions

2018-2020 Duchenne Parent Project. Evaluating a sulforaphane-based nutraceutical to alleviate gastrointestinal dysfunction in DMD

2017-2020 NHMRC Project Grant. Therapeutic potential of skeletal muscle plasticity and slow muscle programming for muscular dystrophy

2017-2020 NHMRC Project Grant. A simple method to improve stem cell transplant therapy

2017-2019 Cancer Council Victoria. Using novel Fn14 inhibitory antibodies to treat cardiac cachexia in cancer

2015-2019 ARC Discovery Project. Understanding the cellular cues that direct muscle stem cell specification

Research Opportunities

This research project is available to PhD, Masters by Research, Honours, Master of Biomedical Science, Post Doctor Research students to join as part of their thesis.
Please contact the Research Group Leader to discuss your options.

Research Group

Lynch laboratory: Basic and clinical myology



Faculty Research Themes

Cancer

School Research Themes

Cancer in Biomedicine, Stem Cells, Molecular Mechanisms of Disease



Key Contact

For further information about this research, please contact the research group leader.

Department / Centre

Physiology

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