Post-natal control of cardiac gene regulatory networks

Project Details

Epigenetic modifications are critical for the precise temporal regulation of gene expression during heart development. We are trying to map the changes in the genomic landscape during post-natal heart development to identify the molecular drivers of cardiac regeneration during early developmental stages.

We tailor projects towards the interests of the student with an emphasis on molecular biology approaches, animal models of cardiac regeneration/repair and human disease models using induced pluripotent stem cells. The scope of the projects can be modified to enable sufficient experimental work for Honours, Masters or PhD level degrees.

Research Opportunities

This research project is available to PhD, Masters by Research, Honours students to join as part of their thesis.
Please contact the Research Group Leader to discuss your options.

Research Publications

Mills RJ, Parker BL, Quaife-Ryan GA, Voges HK, Needham EJ, Bornot A, Ding M, Andersson H, Polla M, Elliott DA, Drowley L, Clausen M, Plowright AT, Barrett IP, Wang QD, James DE, Porrello ER#, Hudson JE#. Cell Stem Cell. 2019 Mar 21. Drug Screening in Human PSC-Cardiac Organoids Identifies Pro-proliferative Compounds Acting via the Mevalonate Pathway. pii: S1934-5909(19)30108-0. doi: 10.1016/j.stem.2019.03.009. PMID: 30930147 [Epub ahead of print]. #Co-corresponding

Porrello ER, Mahmoud AI, Simpson E, Hill JA Richardson JA, Olson EN, Sadek H. Transient regenerative potential of the neonatal mouse heart. Science. 2011. 331(6020): 1078-80.

Porrello ER, Johnson BA, Aurora AB, Simpson E, Nam Y-J, Matkovich SJ, Dorn GW, van Rooij E, Olson EN. miR-15 family regulates postnatal mitotic arrest of cardiomyocytes. Circulation Research. 2011. 109(6): 670-9. PMID: 21778430

Porrello ER, Mahmoud AI, Simpson E, Johnson BA, Grinsfelder D, Canseco D, Mammen PP, Rothermel BA, Olson EN, Sadek HA. Regulation of neonatal and adult heart regeneration by the miR-15 family. Proc Natl Acad Sci USA. 2013. 110(1): 187-92. PMID: 23248315

Mahmoud AI, Kocabas, F, Muralidhar S, Kimura W, Koura AS, Thet S, Porrello ER, Sadek HA. Meis1 regulates postnatal cardiomyocyte cell cycle arrest. Nature. 2013. 497(7448): 249-53. PMID: 23594737

Xin M, Kim Y, Sutherland LB, Murakami M, Qi X, McAnally J, Porrello ER, Mahmoud AI, Tan W, Shelton JM, Richardson JA, Sadek HA, Bassel-Duby R, Olson EN. Hippo pathway effector Yap promotes cardiac regeneration. Proc Natl Acad Sci USA. 2013. 110(34): 13839-44. PMID: 23918388

Sim CB, Ziemann M, Kaspi A, Harikrishnan KN, Ooi J, Khurana I, Chang L, Hudson JE, El-Osta A, Porrello ER. Dynamic changes in the cardiac methylome during postnatal development. FASEB J. 2015. 29(4):1329-43. PMID: 25491312

Voges HK, Mills RJ, Elliott DA, Parton RG, Porrello ER, Hudson JE. Development of a human cardiac organoid model reveals innate regenerative potential. Development. 2017. 144(6):1118-1127. PMID: 28174241

Quaife-Ryan GA, Sim CB, Ziemann M, Kaspi A, Rafehi H, Ramialison M, El-Osta A, Hudson JE, Porrello ER. Multi-cellular Transcriptional Analysis of Mammalian Heart Regeneration. Circulation. 2017. 136(12):1123-1139. PMID: 28733351

Mills RJ, Titmarsh DM, Koenig X, Parker BL, Ryall JG, Quaife-Ryan GA, Voges HK, Hodson MP , Ferguson C, Drowley L, Plowright AT, Needham EJ, Wang Q, Gregorevic P, Xin M, Thomas WG, Parton RG, Nielsen LK, Launikonis BS, James DE, Elliott DA, Porrello ER#, Hudson JE#. Functional Screening in Human Cardiac Organoids Reveals a Metabolic Mechanism for Cardiomyocyte Cell Cycle Arrest. Proc Natl Acad Sci USA. 2017. 14(40):E8372-E8381. PMID: 28916735

Mills RJ, Parker BL, Monnot P, Needham EJ, Vivien CJ, Ferguson C, Parton RG, James DE, Porrello ER, Hudson JE. Development of a human skeletal micro muscle platform with pacing capabilities. Biomaterials. 2019. 198: 217-227. PMID: 30527761

Research Group

Porrello laboratory: Cardiac Regeneration


School Research Themes

Cardio-Respiratory, Stem Cells



Key Contact

For further information about this research, please contact the research group leader.

Department / Centre

Physiology

MDHS Research library
Explore by researcher, school, project or topic.