Imaging transcription factor DNA target search in a living cell
Transcription factors have evolved DNA target search strategies that allow them to efficiently navigate the nuclear space and arrive at their specific DNA sequence. This target search strategy is underpinned by molecular diffusion, which in turn is controlled by the architectural organisation of the cell nucleus and oligomeric state of the transcription factor. Until recently no imaging approach could track the molecular mobility of protein oligomers within the nuclei of live cells. To address this research gap we recently established a new microscopy method to image the transport and binding dynamics of different oligomeric species in live cells (Hinde et al. 2016. Nature Comm. 11047). The overall aim of this project is to use to use this technology to uncover how the spatial compartmentalisation of the cell nucleus regulates transcription factor complex formation and DNA target search in a living cell.
Jieqiong Lou, Post doctoral researcher
Julia Ivanova, Masters research student
Professor Enrico Gratton, University of California, Irvine, USA.
Associate Professor Marie Bogoyevitch, University of Melbourne, Australia.
Professor David Jans, Monash University, Australia.
ARC Discovery Project (2018-2021) 'Nuclear architecture in a living cell facilitates navigation of the genome'.
Hinde E, Pandzic E, Yang Z, Ng IHW, Jans DA, Bogoyevitch MA, Gratton E, Gaus K. Quantifying the dynamics of the oligomeric transcription factor STAT3 by pair correlation of molecular brightness. Nature Communications 2016; 7(11047).
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