GridION X5 long-read sequencer

This resource is available to all University of Melbourne researchers and provides the opportunity to use the latest technology in long-read sequencing. We also provide flow cells for purchase at competitive prices in addition to access to the GridION instrument.

ABOUT

This resource is available to all University of Melbourne researchers and provides the opportunity to use the latest technology in long-read sequencing. We also provide flow cells for purchase at competitive prices in addition to access to the GridION instrument.

The GridION X5 offers real-time, long-read, high-fidelity DNA and RNA sequencing. See the link below for further information.

https://nanoporetech.com/products/gridion

The current chemistry and software release (March 2019) enables generation of up to 30 Gb of data per flow cell during a GridION X5 run and the compute module is able to base call in real time.

Prospective users will receive training in GridION use. Contact us for assistance with your long-read sequencing queries.

LOCATION

PC2 Facility located at Room 2.56, Level 2, Melbourne Brain Centre (Building 144, Kenneth Myer Building, University of Melbourne, Royal Parade, Parkville).

Please complete the below items before using the GridION sequencer:

  • Data storage:
    • IF UoM user make sure you have a Mediaflux project with at least 1TB free:
      • To request a Mediaflux project submit a Research Data Storage Request via this link http://go.unimelb.edu.au/z4q6
        • For “Type of storage” select “Research data storage or access to data management platform” and in “ADDITIONAL INFORMATION” field say “Request for Mediaflux”
      • To increase an allocation for an existing project http://go.unimelb.edu.au/zak6
        • For “REQUEST TYPE” select “Research data management” and tell us which Mediaflux project you would like increased and by how much.
    • IF NOT UoM user: Contact us to discuss possibilities.
  • Training:
    • All users MUST be trained on flow cell loading and using the instrument prior to making a booking.
  • Booking:
    • All users MUST have a booking before any sequencing run.

Data retention and clean-up policy:

  • IF UoM user: All sequencing data can be uploaded from the GridION to your nominated destination Project on MediaFlux.
    • You MUST have a MediaFlux account prior to performing your sequencing run with the recommended storage space.
  • IF NOT UoM user: Contact us.

BOOKINGS

Available for booking for short (up to 4 hours) or long runs (up to 48 hours), with flow cell loading occurring during normal University of Melbourne opening hours Monday - Friday (0900 – 1700). Please note that outside of these hours, non-KMB staff will not be able to access the building or PC2 labs.

Please contact us to be provided with a lab link to book GridION slots via Teamup. Current bookings can be viewed by following the link below (read only): https://teamup.com/ksnt5umb5r49kaq15g

DATA ANALYSIS

Note: We do not provide data analysis other than investigating any issues with real-time base-calling or experiment failures during the experiment.

CONTACT US

GridION email: long-reads@unimelb.edu.au

For bookings and invoice enquiries: Dr. Vanta Jameson via vanta.jameson@unimelb.edu.au

Instrument expert/lab head: Dr. Mike Clark via michael.clark@unimelb.edu.au

ACKNOWLEDGEMENT

If appropriate, please acknowledge specific research staff, Dr. Mike Clark, Mr. Ricardo De Paoli-Iseppi or Dr. Vanta Jameson for their assistance during your experimental design or sequencing analysis process.