Introduction to Flow Cytometry Lecture series May 2021
Introduction to UoM Cytometry, Microscopy and Histology Platforms lecture series via Zoom
The lectures are aimed at researchers who are new to the applications of histology, fluorescence microscopy and flow cytometry, or those who would like to refresh their understanding. This event is free to attend and open to all and will be delivered by managers of the respective platforms, Melbourne Histology Platform (MHP), Fluorescence Microscopy Platform (BOMP) and Melbourne Cytometry Platform (MCP)
Lecture 1: Intro to Fluorescence and Fluorescence Microscopy (BOMP)
(10am-12pm Monday 3rd May)
- Fluorescence
- Fluorescence tags, dyes & proteins
- Fluorescence Microscope
Lecture 2: Introduction to Flow Cytometry and examples of applications (MCP)
(10am-12pm Tuesday 4th May)
- Cytometry Platform: Who, what & where?
- Cytometer architecture
- Instrument subsystems: Fluidics, Optics & Electronics
- Nature of the signals (Light Scatter, Fluorescence)
- Pulse processing
- Data Visualisation
- Examples of applications in Cytometry
Lecture 3: Experimental design, acquisition and analysis on Fluorescence Microscopy (BOMP)
(10am-12pm Wednesday 5th May)
- Fluorescence Microscopy Applications
- Experimental design
- Digital imaging and Image analysis
Lecture 4: Experimental design, acquisition & analysis on Flow Cytometry (MCP)
(10am-12pm Friday 7th May)
- Experimental parameters in Cytometry
- Before you start
- Sample preparation
- Instrument setup
- Compensation and spectral unmixing
- Panel Design
- Experimental Controls
- Data acquisition and analysis
- Examples of artefacts
- Cell Sorting
Lecture 5: Introduction to Histology (MHP)
(10am-12pm Monday 10th May)
- The aims of histology
- Overview of the key steps of histology
- Broad options for sample fixation, embedding, sectioning, and staining
- Basic introduction to immunostaining
Lecture 6: Experimental design for and applications in histology (MHP)
(10am-12pm Tuesday 11th May)
- Integrating histology into experimental design
- Technology in sample preparation
- Examples of applications of histology in biomedical and biological research