Acquisition, Epigenetic and Transcriptional Regulation of CTL Gene Expression Within Both Effector and Memory CTL
Upon activation, naïve T cells undergo a program of proliferation and acquisition of lineage specific functions. Importantly, the factors that regulate acquisition and maintenance of T cell effector function. Furthermore, we are particularly interested in how T cell effector functions acquired during initial activation are maintained into memory. We are using a combination of a single cell RT-PCR technique, identifying novel transcriptional and epigenetic pathways, and over-expression analysis of key regulators to map key events that enable lineage specific T cell function and the establishment of memory. Such analysis will tell us if the molecular mechanisms regulating transcriptional control of T cell effector function are unique and/or shared among key effector genes. This will lead to identification of key immune correlates of protective immunity and will contribute to improvement of immunotherapies for both infectious and autoimmune disease.