The role of Hedgehog signalling during ocular morphogenesis
The development of the eye involves inductive interactions between embryonic head ectoderm and neuroectoderm, resulting in formation of the lens and retina. This is followed by migration of mesenchymal cells that populate the developing cornea. Using conditional gene deletion techniques we have generated mice that lack Hedgehog signaling in the ocular ectoderm and found distinct phenotypes in lens and cornea. In addition, these mice also appear to have defective migration of extra-ocular mesenchymal cells into the eye. This project will examine the molecular mechanisms that underlie these phenotypes.
Techniques include breeding and management of mouse colonies, collection of tissues for histology, and investigating molecular markers of ocular development by RT-PCR, immunohistochemistry, western blotting and in situ hybridisation.
A/Prof Robb de Iongh
Faculty Research Themes
School Research Themes
For further information about this research, please contact the research group leader.