Regulation of BDNF/TrkB Signalling by Suppressor of Cytokine Signalling-2 (SOCS2)
SOCS2 negatively regulates multiple signalling pathways, including growth homone, the EGF receptor and the NGF receptor TrkA. We have also shown recently that it interacts with the BDNF receptor TrkB. This current project is examining how this interaction occurs and what effects it has on neuronal growth. We are using a range of molecular techniques to examine which domains of TrkB and SOCS2 are required for their interaction. We are also using transfected primary hippocampal neuron cultures to determine effects of the different SOCS2 constructs on neurite outgrowth, in the presence and absence of BDNF. Finally we are examining the role of SOCS2 in modulating ubiquitination of TrkB and how this affects TrkB localisation and function.