Improving Vaccines: Mechanisms of Vaccine Biology
Understanding the cell biology of antibody-targeted vaccination
Antibody Targeted Vaccination is a novel mode of vaccination currently in clinical trials. Antigen is targeted directly to receptors expressed by immune cells.
Surprisingly little is known about which receptors are the best to target with antigen or how the targeted receptors are internalised and trafficked inside the cell. We are monitoring the trafficking routes of immune cell receptors using innovative DNA-based fluorescent probes (Figure 1).
In addition, we are tracking the intracellular routes of targeted receptor inside dendritic cells. Defining how receptors traffic vaccine antigen inside the cell is important to determine the usefulness of individual receptors as vaccine targets in clinical settings. Finally, we are using a novel proteomics approach to identify new receptors that will serve as effective vaccine targets.
Figure 1: Novel DNA-based fluorescent probe for monitoring receptor endocytosis
Nanoparticles are a new and promising approach to vaccination. We are investigating how nanoparticles can be manipulated to enhance antigen presentation and immunity. To do this, we are investigating the cell biology of different nanoparticle formulations in primary dendritic cells, together with evaluating their effectiveness as vaccines in models of infection in vivo.
Figure 2: Antibody-targeted nanoparticle
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