Designing Better Drugs

Project Details

Identifying and developing new drugs is a time consuming and expensive process, and the attrition rate of the drug design process remains high. Using graph-based signatures we have been developing approaches that enable rapid screening of biological activities, along with their pharmacokinetic, pharmacodynamic and toxicity profiles. This can help identify in early stages molecules of interest to focus development efforts upon, reducing failure risks, costs, time and animal usage.

Molecular signatures of biological activity

We are exploring the chemical signatures of molecules that could be used to treat different cancers and microbial pathogens (including TB, malaria and the most dangerous hospital-acquired, antibiotic-resistant infections: Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA), as well as the common causes of fungal infections Cryptococcus neoformans and Candida albicans).

By mapping the chemical signatures of active and inactive molecules, we are generating novel predictive algorithms capable of identifying potential new antibiotics and chemotherapeutics. In collaboration with our partners, these predicted molecules will be tested in models of the diseases.

Small molecules for BIG targets - Targeting protein-protein interactions with fragments

Most proteins work within a network of interactions with other proteins, selectively targeting specific interactions, modulating protein function and providing the opportunity to develop more selective and effective drugs.

But while drugs are usually around 100 Å2, proteins interact tightly using way larger protein-protein interfaces, ranging from 1000-6000 Å2. This raises the challenge of how we can use a small molecule to affect an interface many times larger, which until recently was considered to be flat and undruggable. We and others have had success using fragment-based drug discovery to identify novel protein-protein interaction modulators. This allows us to take advantage of hot-spots within the protein interfaces that mediate a large proportion of the binding energy, growing the molecule to improve binding affinity and drug like properties.

The crystal structures of many protein interface modulators with their targets have been solved, which opens up the possibility for us to ask: what are the major components of binding affinity? and can we use this information to predict fragments likely to bind to a given interface? We are addressing these questions using structural bioinformatics and machine learning, leading to the development of novel programmes, which we validate using biophysical and structural approaches to test fragment binding.

Researchers

Dr David Ascher, Group Leader

Dr Douglas Pires (Fiocruz-Minas)

Carlos Henrique Miranda Rodrigues, Masters Student (UFMG / CPqRR, Brazil), CPNq, co-supervised with Douglas Pires

Collaborators

Professor Sir Tom Blundell, University of Cambridge

Dr Lisa Kaminskas, University of Queensland

Professor Véronique Dartois, Public Health Research Institute Center

Funding

Newton Fund/MRC: "Understanding Antimicrobial Resistance Mutations in Tuberculosis: Towards Personalised Treatment to Combat Multi‐drug Resistance."

Research Opportunities

This research project is available to PhD students to join as part of their thesis.
Please contact the Research Group Leader to discuss your options.

Research Publications

  1. Jubb HC, Higuerueloa AP, Ochoa-Montañoa B, Pittb, WR, Ascher DB, Blundell TL.  Arpeggio: a web server for calculating and visualising interatomic interactions in protein structures. Journal of Molecular Biology 2016; In Press.
  2. Park Y, Pacitto A, Bayliss T, Cleghorn LAT, Wang Z, Hartman T, Arora K, Ioerger TR, Sacchettini J, Rizzi M, Donini S, Blundell TL, Ascher DB, Rhee K, Breda A, Zhou N, Dartois V, Jonnala SR, Via LE, Mizrahi V, Epemolu O, Stojanovski L, Simeons F, Osuna-Cabello M, Ellis L, MacKenzie CJ, Smith ARC, Davis SH, Murugesan D, Buchanan KI, Turner PA, Huggett M, Zuccotto F, Rebollo-Lopez MJ, Lafuente-Monasterio MJ, Sanz O, Diaz GS, Lelièvre J, Ballell L, Selenski C, Axtman M, Ghidelli-Disse S, Pflaumer H, Bösche M, Drewes G, Freiberg GM, Kurnick MD, Srikumaran M, Kempf DJ, Green SR, Ray PC, Read K, Wyatt P, Barry III CE , Boshoff HI. Essential but not vulnerable: indazole sulfonamides targeting inosine monophosphate dehydrogenase as potential leads against Mycobacterium tuberculosis. ACS Infectious Diseases 2016; In Press.
  3. Pires DEV, Ascher DB.  CSM-lig: a web server for assessing and comparing protein-small molecule affinities. Nucleic Acids Research 2016; 44: W557-561.
  4. Singh V, Donini S, Pacitto A, Sala C, Hartkoorn RC, Dhar N, Keri G, Ascher DB, Mondésert G, Vocat A, Lupien A, Sommer R, Vermet H, Lagrange S, Buechler J, Warner DF, McKinney JD, Pato J, Cole ST, Blundell TL, Rizzi M, Mizrahi V.  The inosine monophosphate dehydrogenase, GuaB2, is a vulnerable new bactericidal drug target for tuberculosis. ACS Infectious Diseases 2016; In Press.
  5. Pires DEV, Blundell TL, Ascher DB.  pkCSM: predicting small-molecule pharmacokinetic properties using graph-based signatures. Journal of Medicinal Chemistry 2015; 58(9): 4066-4072.
  6. Sigurdardottir AG, Winter A, Sobkowicz A, Fragai M, Chirgadze D, Ascher DB, Blundell TL, Gherardi E. Exploring the chemical space of the lysine-binding pocket of the first kringle domain of hepatocyte growth factor/scatter factor (HGF/SF) yields a new class of inhibitors of HGF/SF-MET binding. Chemical Science 2015; 6(11): 6147-6157.

Research Group

Ascher Laboratory: Structural Biology and Bioinformatics



Faculty Research Themes

Cancer, Infection and Immunology

School Research Themes

Cancer, Infection & Immunity, Cellular Imaging & Structural Biology, Therapeutics & Translation



Key Contact

For further information about this research, please contact the research group leader.

Department / Centre

Biochemistry and Molecular Biology