Analysers
Hours of Operation: Available to licensed users 24 hours 7 days a week
Cytek Aurora Spectral Cytometer
The Cytek Aurora is equipped with five lasers – UV (355nm), violet (405nm) blue (488nm), yellow (561nm) and red (640nm), three scattering channels, and up to 64 fluorescence detectors. Spectral traces of each fluorochrome are recorded and then unmixed in a highly multicolor experiment. As a result of this approach, combinations of fluorochromes that previously were incompatible to run together by conventional flow cytometric methods are now possible with this technology. As long as a fluorochrome’s spectral fingerprint is distinct from another they can be unmixed. This enables panel design of >36 colours possible.
Cytek Aurora
Lasers: 5 (355nm, 405nm, 488nm, 561nm and 640nm)
Fluorescent Detectors: 64 Channels
Tubes: Polystyrene
Software: Spectroflo
For information on common fluorochromes used on the Aurora click here.
Hours of Operation: Available to licensed users 24 hours 7 days a week
BD LSR Fortessa
The LSR Fortessa’s are equipped with four and five laser lines and are capable of detecting 18 parameters; 2 scatter and 16 fluorescent detectors. Check each instrument for the configuration and the facility staff are happy to advise on fluorochrome combination feasibility and necessary controls.
LSR Fortessa 1
Lasers: 4 (405nm,488nm, 561nm and 633nm)
PMT Detectors: 18 Fluorescent Channels
Tubes: Polystyrene
Software: FACSDiva
High Throughput Sampler (HTS) 96 well plates
Fast Analysis Rates >20,000 events per second
LSR Fortessa 2, 3 and 4
Lasers: 5 (355nm,405nm,488nm, 561nm and 633nm)
PMT Detectors: 18 Fluorescent Channels
Tubes: Polystyrene
Software: FACSDiva
High Throughput Sampler (HTS) 96 well plates
Fast Analysis Rates >20,000 events per second
LSR II
Lasers: 3 (405nm, 488nm, and 633nm)
PMT Detectors: 13 Fluorescent Channels
Tubes: Polystyrene
Software: FACSDiva
Fast Analysis Rates >20,000 events per second
FACS Canto II a
Lasers: 2 (488nm and 633nm)
PMT Detectors: 7 Fluorescent Channels
Tubes: Polystyrene
Software: FACSDiva
Analysis Rate: max 10,000 events per second
FACS Canto II b
Lasers: 3 (405nm,488nm and 633nm)
PMT Detectors: 8 Fluorescent Channels
Tubes: Polystyrene
Software: FACSDiva
High Throughput Sampler (HTS)
Analysis Rate: max 10,000 events per second